Review



human holo-transferrin  (ProSpec)


Bioz Verified Symbol ProSpec is a verified supplier
Bioz Manufacturer Symbol ProSpec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    ProSpec human holo-transferrin
    Human Holo Transferrin, supplied by ProSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+holo-transferrin/human+holo+transferrin/pm39536753-222-133-135
    Average 90 stars, based on 1 article reviews
    human holo-transferrin - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Recombinant:

    Article Title: IL-21/IL-21R signaling renders acute myeloid leukemia stem cells more susceptible to cytarabine treatment and CAR T cell therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6J Charles River MGI: 3028467 Il21R / (B6.129-Il21rtm1Kopf/J) MGI: 5435248 Il21 / (B6.129S-Il21tm1Lex/Mmucd) MGI: 4843320 Il21mCherry(B6.Cg-Tg(Il21-mCherry)1Wjl/Mmnc) MGI: 5478531 Recombinant DNA Plasmid pMDLg/pRRE Addgene #12259 Plasmid pRSV-Rev Addgene #12253 Plasmid pCMV-VSV-g Addgene #8454 CD19-CAR plasmid Addgene #200671 Software and algorithms FlowJoTM software v.10.6 TreeStar N/A GraphPad Prism software v9.0 GraphPad N/A ELDA Software Hu et al.21 http://bioinf.wehi.edu.au/software/elda/ fastqc v. 0.11.9 https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ RSeQC v. 4.0.0 Wang et al.72 N/A HiSat2 v. 2.2.1 Kim et al.73 N/A FeatureCounts v. 2.0.1 Liao et al.74 N/A DESeq2 v.1.32.0 Love et al.75 N/A TopGo v. 2.44.0 https://bioconductor.org/packages/ release/bioc/html/topGO.html ClusterProfiler v. 4.0.2 Yu et al.76 N/A R 4.1.0 https://www.R-project.org/ INSPIRE software https://www.merckmillipore.com/ IDEAS software https://www.merckmillipore.com/ Kaluza analysis software Beckman Coulter Life Sciences N/A Other HALLMARK OXIDATIVE PHOSPHORYLATION gsea-msigdb.org Somervaille_UP Somervaille et al.26 N/A Somervaille_DOWN Somervaille et al.26 N/A STEMNESS_UP gsea-msigdb.org STEMNESS_ DOWN gsea-msigdb.org GAL_LEUKEMIC STEM CELL_DOWN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_DN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_UP gsea-msigdb.org HALLMARK_TNFA_SIGNALING_VIA_NFKB gsea-msigdb.org HSC Differentiation and Lineage-specific markers pathcards.genecards.org Wnt Signaling (beta-Catenin) pathcards.genecards.org MAPK-Erk Signaling pathcards.genecards.org POSITIVE REGULATION OF CELL CYCLE gsea-msigdb.org Respiratory electron transport_ATP synthesis pathcards.genecards.org FRIDMAN_SENESCENCE_UP gsea-msigdb.org TANG_SENESCENCE_TP53_TARGETS_DN gsea-msigdb.org

    Article Title: Genetic modifications of EGLN1 reactivate HbF production in β 0 -thalassemia/HbE.
    Article Snippet: A total of 2 × 105 HSPCs were transfected with Cas9-sgRNA ribonucleoprotein and maintained in the expansion medium for 48 h. HSPCs were subsequently transferred into erythroid differentiation medium (EDM) consisting of IMDM (SH30259.02; Cytiva, Logan, UT) supplemented with 5%v/v male AB serum, 100 U/ml penicillin and 100 μg/ml streptomycin, 2 mM GlutaMAX, 330 μg/ml human holo-transferrin (Prospec-Tany, Ness Ziona, Israel), 10 μg/ml recombinant human insulin (Prospec-Tany), 2 IU/ml heparin (Leo, Ballerup, Denmark) and 3 IU/ml erythropoietin (Cilag AG, Schaffhausen, Switzerland).

    Article Title: Identification of a Novel Neurotrophic Factor from Primary Retinal Müller Cells Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
    Article Snippet: Human recombinant CXCL10 (100 ng/ml; Prospec, Rechovot, Israel), human Holo-transferrin (1000 ng/ml; Prospec), human recombinant LIF (1 and 100 ng/ml; Prospec), human recombinant CCL5 (2 and 10 ng/ml; Peprotec, Hamburg, Germany), and human recombinant IL8 (10 and 100 ng/ml; Peprotec) in DMEM/F-12 medium (Invitrogen) or medium alone (negative control) were applied to the PR cultures 20 h after preparation.


    Virus:

    Article Title: IL-21/IL-21R signaling renders acute myeloid leukemia stem cells more susceptible to cytarabine treatment and CAR T cell therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6J Charles River MGI: 3028467 Il21R / (B6.129-Il21rtm1Kopf/J) MGI: 5435248 Il21 / (B6.129S-Il21tm1Lex/Mmucd) MGI: 4843320 Il21mCherry(B6.Cg-Tg(Il21-mCherry)1Wjl/Mmnc) MGI: 5478531 Recombinant DNA Plasmid pMDLg/pRRE Addgene #12259 Plasmid pRSV-Rev Addgene #12253 Plasmid pCMV-VSV-g Addgene #8454 CD19-CAR plasmid Addgene #200671 Software and algorithms FlowJoTM software v.10.6 TreeStar N/A GraphPad Prism software v9.0 GraphPad N/A ELDA Software Hu et al.21 http://bioinf.wehi.edu.au/software/elda/ fastqc v. 0.11.9 https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ RSeQC v. 4.0.0 Wang et al.72 N/A HiSat2 v. 2.2.1 Kim et al.73 N/A FeatureCounts v. 2.0.1 Liao et al.74 N/A DESeq2 v.1.32.0 Love et al.75 N/A TopGo v. 2.44.0 https://bioconductor.org/packages/ release/bioc/html/topGO.html ClusterProfiler v. 4.0.2 Yu et al.76 N/A R 4.1.0 https://www.R-project.org/ INSPIRE software https://www.merckmillipore.com/ IDEAS software https://www.merckmillipore.com/ Kaluza analysis software Beckman Coulter Life Sciences N/A Other HALLMARK OXIDATIVE PHOSPHORYLATION gsea-msigdb.org Somervaille_UP Somervaille et al.26 N/A Somervaille_DOWN Somervaille et al.26 N/A STEMNESS_UP gsea-msigdb.org STEMNESS_ DOWN gsea-msigdb.org GAL_LEUKEMIC STEM CELL_DOWN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_DN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_UP gsea-msigdb.org HALLMARK_TNFA_SIGNALING_VIA_NFKB gsea-msigdb.org HSC Differentiation and Lineage-specific markers pathcards.genecards.org Wnt Signaling (beta-Catenin) pathcards.genecards.org MAPK-Erk Signaling pathcards.genecards.org POSITIVE REGULATION OF CELL CYCLE gsea-msigdb.org Respiratory electron transport_ATP synthesis pathcards.genecards.org FRIDMAN_SENESCENCE_UP gsea-msigdb.org TANG_SENESCENCE_TP53_TARGETS_DN gsea-msigdb.org

    Article Title: Genetic modifications of EGLN1 reactivate HbF production in β 0 -thalassemia/HbE.
    Article Snippet: A total of 2 × 105 HSPCs were transfected with Cas9-sgRNA ribonucleoprotein and maintained in the expansion medium for 48 h. HSPCs were subsequently transferred into erythroid differentiation medium (EDM) consisting of IMDM (SH30259.02; Cytiva, Logan, UT) supplemented with 5%v/v male AB serum, 100 U/ml penicillin and 100 μg/ml streptomycin, 2 mM GlutaMAX, 330 μg/ml human holo-transferrin (Prospec-Tany, Ness Ziona, Israel), 10 μg/ml recombinant human insulin (Prospec-Tany), 2 IU/ml heparin (Leo, Ballerup, Denmark) and 3 IU/ml erythropoietin (Cilag AG, Schaffhausen, Switzerland).

    Article Title: Identification of a Novel Neurotrophic Factor from Primary Retinal Müller Cells Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
    Article Snippet: Human recombinant CXCL10 (100 ng/ml; Prospec, Rechovot, Israel), human Holo-transferrin (1000 ng/ml; Prospec), human recombinant LIF (1 and 100 ng/ml; Prospec), human recombinant CCL5 (2 and 10 ng/ml; Peprotec, Hamburg, Germany), and human recombinant IL8 (10 and 100 ng/ml; Peprotec) in DMEM/F-12 medium (Invitrogen) or medium alone (negative control) were applied to the PR cultures 20 h after preparation.


    Enzyme-linked Immunosorbent Assay:

    Article Title: IL-21/IL-21R signaling renders acute myeloid leukemia stem cells more susceptible to cytarabine treatment and CAR T cell therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6J Charles River MGI: 3028467 Il21R / (B6.129-Il21rtm1Kopf/J) MGI: 5435248 Il21 / (B6.129S-Il21tm1Lex/Mmucd) MGI: 4843320 Il21mCherry(B6.Cg-Tg(Il21-mCherry)1Wjl/Mmnc) MGI: 5478531 Recombinant DNA Plasmid pMDLg/pRRE Addgene #12259 Plasmid pRSV-Rev Addgene #12253 Plasmid pCMV-VSV-g Addgene #8454 CD19-CAR plasmid Addgene #200671 Software and algorithms FlowJoTM software v.10.6 TreeStar N/A GraphPad Prism software v9.0 GraphPad N/A ELDA Software Hu et al.21 http://bioinf.wehi.edu.au/software/elda/ fastqc v. 0.11.9 https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ RSeQC v. 4.0.0 Wang et al.72 N/A HiSat2 v. 2.2.1 Kim et al.73 N/A FeatureCounts v. 2.0.1 Liao et al.74 N/A DESeq2 v.1.32.0 Love et al.75 N/A TopGo v. 2.44.0 https://bioconductor.org/packages/ release/bioc/html/topGO.html ClusterProfiler v. 4.0.2 Yu et al.76 N/A R 4.1.0 https://www.R-project.org/ INSPIRE software https://www.merckmillipore.com/ IDEAS software https://www.merckmillipore.com/ Kaluza analysis software Beckman Coulter Life Sciences N/A Other HALLMARK OXIDATIVE PHOSPHORYLATION gsea-msigdb.org Somervaille_UP Somervaille et al.26 N/A Somervaille_DOWN Somervaille et al.26 N/A STEMNESS_UP gsea-msigdb.org STEMNESS_ DOWN gsea-msigdb.org GAL_LEUKEMIC STEM CELL_DOWN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_DN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_UP gsea-msigdb.org HALLMARK_TNFA_SIGNALING_VIA_NFKB gsea-msigdb.org HSC Differentiation and Lineage-specific markers pathcards.genecards.org Wnt Signaling (beta-Catenin) pathcards.genecards.org MAPK-Erk Signaling pathcards.genecards.org POSITIVE REGULATION OF CELL CYCLE gsea-msigdb.org Respiratory electron transport_ATP synthesis pathcards.genecards.org FRIDMAN_SENESCENCE_UP gsea-msigdb.org TANG_SENESCENCE_TP53_TARGETS_DN gsea-msigdb.org

    Article Title: Genetic modifications of EGLN1 reactivate HbF production in β 0 -thalassemia/HbE.
    Article Snippet: A total of 2 × 105 HSPCs were transfected with Cas9-sgRNA ribonucleoprotein and maintained in the expansion medium for 48 h. HSPCs were subsequently transferred into erythroid differentiation medium (EDM) consisting of IMDM (SH30259.02; Cytiva, Logan, UT) supplemented with 5%v/v male AB serum, 100 U/ml penicillin and 100 μg/ml streptomycin, 2 mM GlutaMAX, 330 μg/ml human holo-transferrin (Prospec-Tany, Ness Ziona, Israel), 10 μg/ml recombinant human insulin (Prospec-Tany), 2 IU/ml heparin (Leo, Ballerup, Denmark) and 3 IU/ml erythropoietin (Cilag AG, Schaffhausen, Switzerland).

    Article Title: Identification of a Novel Neurotrophic Factor from Primary Retinal Müller Cells Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
    Article Snippet: Human recombinant CXCL10 (100 ng/ml; Prospec, Rechovot, Israel), human Holo-transferrin (1000 ng/ml; Prospec), human recombinant LIF (1 and 100 ng/ml; Prospec), human recombinant CCL5 (2 and 10 ng/ml; Peprotec, Hamburg, Germany), and human recombinant IL8 (10 and 100 ng/ml; Peprotec) in DMEM/F-12 medium (Invitrogen) or medium alone (negative control) were applied to the PR cultures 20 h after preparation.


    Enzyme-linked Immunospot:

    Article Title: IL-21/IL-21R signaling renders acute myeloid leukemia stem cells more susceptible to cytarabine treatment and CAR T cell therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6J Charles River MGI: 3028467 Il21R / (B6.129-Il21rtm1Kopf/J) MGI: 5435248 Il21 / (B6.129S-Il21tm1Lex/Mmucd) MGI: 4843320 Il21mCherry(B6.Cg-Tg(Il21-mCherry)1Wjl/Mmnc) MGI: 5478531 Recombinant DNA Plasmid pMDLg/pRRE Addgene #12259 Plasmid pRSV-Rev Addgene #12253 Plasmid pCMV-VSV-g Addgene #8454 CD19-CAR plasmid Addgene #200671 Software and algorithms FlowJoTM software v.10.6 TreeStar N/A GraphPad Prism software v9.0 GraphPad N/A ELDA Software Hu et al.21 http://bioinf.wehi.edu.au/software/elda/ fastqc v. 0.11.9 https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ RSeQC v. 4.0.0 Wang et al.72 N/A HiSat2 v. 2.2.1 Kim et al.73 N/A FeatureCounts v. 2.0.1 Liao et al.74 N/A DESeq2 v.1.32.0 Love et al.75 N/A TopGo v. 2.44.0 https://bioconductor.org/packages/ release/bioc/html/topGO.html ClusterProfiler v. 4.0.2 Yu et al.76 N/A R 4.1.0 https://www.R-project.org/ INSPIRE software https://www.merckmillipore.com/ IDEAS software https://www.merckmillipore.com/ Kaluza analysis software Beckman Coulter Life Sciences N/A Other HALLMARK OXIDATIVE PHOSPHORYLATION gsea-msigdb.org Somervaille_UP Somervaille et al.26 N/A Somervaille_DOWN Somervaille et al.26 N/A STEMNESS_UP gsea-msigdb.org STEMNESS_ DOWN gsea-msigdb.org GAL_LEUKEMIC STEM CELL_DOWN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_DN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_UP gsea-msigdb.org HALLMARK_TNFA_SIGNALING_VIA_NFKB gsea-msigdb.org HSC Differentiation and Lineage-specific markers pathcards.genecards.org Wnt Signaling (beta-Catenin) pathcards.genecards.org MAPK-Erk Signaling pathcards.genecards.org POSITIVE REGULATION OF CELL CYCLE gsea-msigdb.org Respiratory electron transport_ATP synthesis pathcards.genecards.org FRIDMAN_SENESCENCE_UP gsea-msigdb.org TANG_SENESCENCE_TP53_TARGETS_DN gsea-msigdb.org

    Article Title: Genetic modifications of EGLN1 reactivate HbF production in β 0 -thalassemia/HbE.
    Article Snippet: A total of 2 × 105 HSPCs were transfected with Cas9-sgRNA ribonucleoprotein and maintained in the expansion medium for 48 h. HSPCs were subsequently transferred into erythroid differentiation medium (EDM) consisting of IMDM (SH30259.02; Cytiva, Logan, UT) supplemented with 5%v/v male AB serum, 100 U/ml penicillin and 100 μg/ml streptomycin, 2 mM GlutaMAX, 330 μg/ml human holo-transferrin (Prospec-Tany, Ness Ziona, Israel), 10 μg/ml recombinant human insulin (Prospec-Tany), 2 IU/ml heparin (Leo, Ballerup, Denmark) and 3 IU/ml erythropoietin (Cilag AG, Schaffhausen, Switzerland).

    Article Title: Identification of a Novel Neurotrophic Factor from Primary Retinal Müller Cells Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
    Article Snippet: Human recombinant CXCL10 (100 ng/ml; Prospec, Rechovot, Israel), human Holo-transferrin (1000 ng/ml; Prospec), human recombinant LIF (1 and 100 ng/ml; Prospec), human recombinant CCL5 (2 and 10 ng/ml; Peprotec, Hamburg, Germany), and human recombinant IL8 (10 and 100 ng/ml; Peprotec) in DMEM/F-12 medium (Invitrogen) or medium alone (negative control) were applied to the PR cultures 20 h after preparation.


    RNA Sequencing Assay:

    Article Title: IL-21/IL-21R signaling renders acute myeloid leukemia stem cells more susceptible to cytarabine treatment and CAR T cell therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6J Charles River MGI: 3028467 Il21R / (B6.129-Il21rtm1Kopf/J) MGI: 5435248 Il21 / (B6.129S-Il21tm1Lex/Mmucd) MGI: 4843320 Il21mCherry(B6.Cg-Tg(Il21-mCherry)1Wjl/Mmnc) MGI: 5478531 Recombinant DNA Plasmid pMDLg/pRRE Addgene #12259 Plasmid pRSV-Rev Addgene #12253 Plasmid pCMV-VSV-g Addgene #8454 CD19-CAR plasmid Addgene #200671 Software and algorithms FlowJoTM software v.10.6 TreeStar N/A GraphPad Prism software v9.0 GraphPad N/A ELDA Software Hu et al.21 http://bioinf.wehi.edu.au/software/elda/ fastqc v. 0.11.9 https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ RSeQC v. 4.0.0 Wang et al.72 N/A HiSat2 v. 2.2.1 Kim et al.73 N/A FeatureCounts v. 2.0.1 Liao et al.74 N/A DESeq2 v.1.32.0 Love et al.75 N/A TopGo v. 2.44.0 https://bioconductor.org/packages/ release/bioc/html/topGO.html ClusterProfiler v. 4.0.2 Yu et al.76 N/A R 4.1.0 https://www.R-project.org/ INSPIRE software https://www.merckmillipore.com/ IDEAS software https://www.merckmillipore.com/ Kaluza analysis software Beckman Coulter Life Sciences N/A Other HALLMARK OXIDATIVE PHOSPHORYLATION gsea-msigdb.org Somervaille_UP Somervaille et al.26 N/A Somervaille_DOWN Somervaille et al.26 N/A STEMNESS_UP gsea-msigdb.org STEMNESS_ DOWN gsea-msigdb.org GAL_LEUKEMIC STEM CELL_DOWN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_DN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_UP gsea-msigdb.org HALLMARK_TNFA_SIGNALING_VIA_NFKB gsea-msigdb.org HSC Differentiation and Lineage-specific markers pathcards.genecards.org Wnt Signaling (beta-Catenin) pathcards.genecards.org MAPK-Erk Signaling pathcards.genecards.org POSITIVE REGULATION OF CELL CYCLE gsea-msigdb.org Respiratory electron transport_ATP synthesis pathcards.genecards.org FRIDMAN_SENESCENCE_UP gsea-msigdb.org TANG_SENESCENCE_TP53_TARGETS_DN gsea-msigdb.org

    Article Title: Genetic modifications of EGLN1 reactivate HbF production in β 0 -thalassemia/HbE.
    Article Snippet: A total of 2 × 105 HSPCs were transfected with Cas9-sgRNA ribonucleoprotein and maintained in the expansion medium for 48 h. HSPCs were subsequently transferred into erythroid differentiation medium (EDM) consisting of IMDM (SH30259.02; Cytiva, Logan, UT) supplemented with 5%v/v male AB serum, 100 U/ml penicillin and 100 μg/ml streptomycin, 2 mM GlutaMAX, 330 μg/ml human holo-transferrin (Prospec-Tany, Ness Ziona, Israel), 10 μg/ml recombinant human insulin (Prospec-Tany), 2 IU/ml heparin (Leo, Ballerup, Denmark) and 3 IU/ml erythropoietin (Cilag AG, Schaffhausen, Switzerland).

    Article Title: Identification of a Novel Neurotrophic Factor from Primary Retinal Müller Cells Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
    Article Snippet: Human recombinant CXCL10 (100 ng/ml; Prospec, Rechovot, Israel), human Holo-transferrin (1000 ng/ml; Prospec), human recombinant LIF (1 and 100 ng/ml; Prospec), human recombinant CCL5 (2 and 10 ng/ml; Peprotec, Hamburg, Germany), and human recombinant IL8 (10 and 100 ng/ml; Peprotec) in DMEM/F-12 medium (Invitrogen) or medium alone (negative control) were applied to the PR cultures 20 h after preparation.


    Control:

    Article Title: IL-21/IL-21R signaling renders acute myeloid leukemia stem cells more susceptible to cytarabine treatment and CAR T cell therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6J Charles River MGI: 3028467 Il21R / (B6.129-Il21rtm1Kopf/J) MGI: 5435248 Il21 / (B6.129S-Il21tm1Lex/Mmucd) MGI: 4843320 Il21mCherry(B6.Cg-Tg(Il21-mCherry)1Wjl/Mmnc) MGI: 5478531 Recombinant DNA Plasmid pMDLg/pRRE Addgene #12259 Plasmid pRSV-Rev Addgene #12253 Plasmid pCMV-VSV-g Addgene #8454 CD19-CAR plasmid Addgene #200671 Software and algorithms FlowJoTM software v.10.6 TreeStar N/A GraphPad Prism software v9.0 GraphPad N/A ELDA Software Hu et al.21 http://bioinf.wehi.edu.au/software/elda/ fastqc v. 0.11.9 https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ RSeQC v. 4.0.0 Wang et al.72 N/A HiSat2 v. 2.2.1 Kim et al.73 N/A FeatureCounts v. 2.0.1 Liao et al.74 N/A DESeq2 v.1.32.0 Love et al.75 N/A TopGo v. 2.44.0 https://bioconductor.org/packages/ release/bioc/html/topGO.html ClusterProfiler v. 4.0.2 Yu et al.76 N/A R 4.1.0 https://www.R-project.org/ INSPIRE software https://www.merckmillipore.com/ IDEAS software https://www.merckmillipore.com/ Kaluza analysis software Beckman Coulter Life Sciences N/A Other HALLMARK OXIDATIVE PHOSPHORYLATION gsea-msigdb.org Somervaille_UP Somervaille et al.26 N/A Somervaille_DOWN Somervaille et al.26 N/A STEMNESS_UP gsea-msigdb.org STEMNESS_ DOWN gsea-msigdb.org GAL_LEUKEMIC STEM CELL_DOWN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_DN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_UP gsea-msigdb.org HALLMARK_TNFA_SIGNALING_VIA_NFKB gsea-msigdb.org HSC Differentiation and Lineage-specific markers pathcards.genecards.org Wnt Signaling (beta-Catenin) pathcards.genecards.org MAPK-Erk Signaling pathcards.genecards.org POSITIVE REGULATION OF CELL CYCLE gsea-msigdb.org Respiratory electron transport_ATP synthesis pathcards.genecards.org FRIDMAN_SENESCENCE_UP gsea-msigdb.org TANG_SENESCENCE_TP53_TARGETS_DN gsea-msigdb.org

    Article Title: Genetic modifications of EGLN1 reactivate HbF production in β 0 -thalassemia/HbE.
    Article Snippet: A total of 2 × 105 HSPCs were transfected with Cas9-sgRNA ribonucleoprotein and maintained in the expansion medium for 48 h. HSPCs were subsequently transferred into erythroid differentiation medium (EDM) consisting of IMDM (SH30259.02; Cytiva, Logan, UT) supplemented with 5%v/v male AB serum, 100 U/ml penicillin and 100 μg/ml streptomycin, 2 mM GlutaMAX, 330 μg/ml human holo-transferrin (Prospec-Tany, Ness Ziona, Israel), 10 μg/ml recombinant human insulin (Prospec-Tany), 2 IU/ml heparin (Leo, Ballerup, Denmark) and 3 IU/ml erythropoietin (Cilag AG, Schaffhausen, Switzerland).

    Article Title: Identification of a Novel Neurotrophic Factor from Primary Retinal Müller Cells Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
    Article Snippet: Human recombinant CXCL10 (100 ng/ml; Prospec, Rechovot, Israel), human Holo-transferrin (1000 ng/ml; Prospec), human recombinant LIF (1 and 100 ng/ml; Prospec), human recombinant CCL5 (2 and 10 ng/ml; Peprotec, Hamburg, Germany), and human recombinant IL8 (10 and 100 ng/ml; Peprotec) in DMEM/F-12 medium (Invitrogen) or medium alone (negative control) were applied to the PR cultures 20 h after preparation.


    Plasmid Preparation:

    Article Title: IL-21/IL-21R signaling renders acute myeloid leukemia stem cells more susceptible to cytarabine treatment and CAR T cell therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6J Charles River MGI: 3028467 Il21R / (B6.129-Il21rtm1Kopf/J) MGI: 5435248 Il21 / (B6.129S-Il21tm1Lex/Mmucd) MGI: 4843320 Il21mCherry(B6.Cg-Tg(Il21-mCherry)1Wjl/Mmnc) MGI: 5478531 Recombinant DNA Plasmid pMDLg/pRRE Addgene #12259 Plasmid pRSV-Rev Addgene #12253 Plasmid pCMV-VSV-g Addgene #8454 CD19-CAR plasmid Addgene #200671 Software and algorithms FlowJoTM software v.10.6 TreeStar N/A GraphPad Prism software v9.0 GraphPad N/A ELDA Software Hu et al.21 http://bioinf.wehi.edu.au/software/elda/ fastqc v. 0.11.9 https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ RSeQC v. 4.0.0 Wang et al.72 N/A HiSat2 v. 2.2.1 Kim et al.73 N/A FeatureCounts v. 2.0.1 Liao et al.74 N/A DESeq2 v.1.32.0 Love et al.75 N/A TopGo v. 2.44.0 https://bioconductor.org/packages/ release/bioc/html/topGO.html ClusterProfiler v. 4.0.2 Yu et al.76 N/A R 4.1.0 https://www.R-project.org/ INSPIRE software https://www.merckmillipore.com/ IDEAS software https://www.merckmillipore.com/ Kaluza analysis software Beckman Coulter Life Sciences N/A Other HALLMARK OXIDATIVE PHOSPHORYLATION gsea-msigdb.org Somervaille_UP Somervaille et al.26 N/A Somervaille_DOWN Somervaille et al.26 N/A STEMNESS_UP gsea-msigdb.org STEMNESS_ DOWN gsea-msigdb.org GAL_LEUKEMIC STEM CELL_DOWN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_DN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_UP gsea-msigdb.org HALLMARK_TNFA_SIGNALING_VIA_NFKB gsea-msigdb.org HSC Differentiation and Lineage-specific markers pathcards.genecards.org Wnt Signaling (beta-Catenin) pathcards.genecards.org MAPK-Erk Signaling pathcards.genecards.org POSITIVE REGULATION OF CELL CYCLE gsea-msigdb.org Respiratory electron transport_ATP synthesis pathcards.genecards.org FRIDMAN_SENESCENCE_UP gsea-msigdb.org TANG_SENESCENCE_TP53_TARGETS_DN gsea-msigdb.org

    Article Title: Genetic modifications of EGLN1 reactivate HbF production in β 0 -thalassemia/HbE.
    Article Snippet: A total of 2 × 105 HSPCs were transfected with Cas9-sgRNA ribonucleoprotein and maintained in the expansion medium for 48 h. HSPCs were subsequently transferred into erythroid differentiation medium (EDM) consisting of IMDM (SH30259.02; Cytiva, Logan, UT) supplemented with 5%v/v male AB serum, 100 U/ml penicillin and 100 μg/ml streptomycin, 2 mM GlutaMAX, 330 μg/ml human holo-transferrin (Prospec-Tany, Ness Ziona, Israel), 10 μg/ml recombinant human insulin (Prospec-Tany), 2 IU/ml heparin (Leo, Ballerup, Denmark) and 3 IU/ml erythropoietin (Cilag AG, Schaffhausen, Switzerland).

    Article Title: Identification of a Novel Neurotrophic Factor from Primary Retinal Müller Cells Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
    Article Snippet: Human recombinant CXCL10 (100 ng/ml; Prospec, Rechovot, Israel), human Holo-transferrin (1000 ng/ml; Prospec), human recombinant LIF (1 and 100 ng/ml; Prospec), human recombinant CCL5 (2 and 10 ng/ml; Peprotec, Hamburg, Germany), and human recombinant IL8 (10 and 100 ng/ml; Peprotec) in DMEM/F-12 medium (Invitrogen) or medium alone (negative control) were applied to the PR cultures 20 h after preparation.


    Software:

    Article Title: IL-21/IL-21R signaling renders acute myeloid leukemia stem cells more susceptible to cytarabine treatment and CAR T cell therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6J Charles River MGI: 3028467 Il21R / (B6.129-Il21rtm1Kopf/J) MGI: 5435248 Il21 / (B6.129S-Il21tm1Lex/Mmucd) MGI: 4843320 Il21mCherry(B6.Cg-Tg(Il21-mCherry)1Wjl/Mmnc) MGI: 5478531 Recombinant DNA Plasmid pMDLg/pRRE Addgene #12259 Plasmid pRSV-Rev Addgene #12253 Plasmid pCMV-VSV-g Addgene #8454 CD19-CAR plasmid Addgene #200671 Software and algorithms FlowJoTM software v.10.6 TreeStar N/A GraphPad Prism software v9.0 GraphPad N/A ELDA Software Hu et al.21 http://bioinf.wehi.edu.au/software/elda/ fastqc v. 0.11.9 https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ RSeQC v. 4.0.0 Wang et al.72 N/A HiSat2 v. 2.2.1 Kim et al.73 N/A FeatureCounts v. 2.0.1 Liao et al.74 N/A DESeq2 v.1.32.0 Love et al.75 N/A TopGo v. 2.44.0 https://bioconductor.org/packages/ release/bioc/html/topGO.html ClusterProfiler v. 4.0.2 Yu et al.76 N/A R 4.1.0 https://www.R-project.org/ INSPIRE software https://www.merckmillipore.com/ IDEAS software https://www.merckmillipore.com/ Kaluza analysis software Beckman Coulter Life Sciences N/A Other HALLMARK OXIDATIVE PHOSPHORYLATION gsea-msigdb.org Somervaille_UP Somervaille et al.26 N/A Somervaille_DOWN Somervaille et al.26 N/A STEMNESS_UP gsea-msigdb.org STEMNESS_ DOWN gsea-msigdb.org GAL_LEUKEMIC STEM CELL_DOWN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_DN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_UP gsea-msigdb.org HALLMARK_TNFA_SIGNALING_VIA_NFKB gsea-msigdb.org HSC Differentiation and Lineage-specific markers pathcards.genecards.org Wnt Signaling (beta-Catenin) pathcards.genecards.org MAPK-Erk Signaling pathcards.genecards.org POSITIVE REGULATION OF CELL CYCLE gsea-msigdb.org Respiratory electron transport_ATP synthesis pathcards.genecards.org FRIDMAN_SENESCENCE_UP gsea-msigdb.org TANG_SENESCENCE_TP53_TARGETS_DN gsea-msigdb.org

    Article Title: Genetic modifications of EGLN1 reactivate HbF production in β 0 -thalassemia/HbE.
    Article Snippet: A total of 2 × 105 HSPCs were transfected with Cas9-sgRNA ribonucleoprotein and maintained in the expansion medium for 48 h. HSPCs were subsequently transferred into erythroid differentiation medium (EDM) consisting of IMDM (SH30259.02; Cytiva, Logan, UT) supplemented with 5%v/v male AB serum, 100 U/ml penicillin and 100 μg/ml streptomycin, 2 mM GlutaMAX, 330 μg/ml human holo-transferrin (Prospec-Tany, Ness Ziona, Israel), 10 μg/ml recombinant human insulin (Prospec-Tany), 2 IU/ml heparin (Leo, Ballerup, Denmark) and 3 IU/ml erythropoietin (Cilag AG, Schaffhausen, Switzerland).

    Article Title: Identification of a Novel Neurotrophic Factor from Primary Retinal Müller Cells Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
    Article Snippet: Human recombinant CXCL10 (100 ng/ml; Prospec, Rechovot, Israel), human Holo-transferrin (1000 ng/ml; Prospec), human recombinant LIF (1 and 100 ng/ml; Prospec), human recombinant CCL5 (2 and 10 ng/ml; Peprotec, Hamburg, Germany), and human recombinant IL8 (10 and 100 ng/ml; Peprotec) in DMEM/F-12 medium (Invitrogen) or medium alone (negative control) were applied to the PR cultures 20 h after preparation.


    Phospho-proteomics:

    Article Title: IL-21/IL-21R signaling renders acute myeloid leukemia stem cells more susceptible to cytarabine treatment and CAR T cell therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6J Charles River MGI: 3028467 Il21R / (B6.129-Il21rtm1Kopf/J) MGI: 5435248 Il21 / (B6.129S-Il21tm1Lex/Mmucd) MGI: 4843320 Il21mCherry(B6.Cg-Tg(Il21-mCherry)1Wjl/Mmnc) MGI: 5478531 Recombinant DNA Plasmid pMDLg/pRRE Addgene #12259 Plasmid pRSV-Rev Addgene #12253 Plasmid pCMV-VSV-g Addgene #8454 CD19-CAR plasmid Addgene #200671 Software and algorithms FlowJoTM software v.10.6 TreeStar N/A GraphPad Prism software v9.0 GraphPad N/A ELDA Software Hu et al.21 http://bioinf.wehi.edu.au/software/elda/ fastqc v. 0.11.9 https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ RSeQC v. 4.0.0 Wang et al.72 N/A HiSat2 v. 2.2.1 Kim et al.73 N/A FeatureCounts v. 2.0.1 Liao et al.74 N/A DESeq2 v.1.32.0 Love et al.75 N/A TopGo v. 2.44.0 https://bioconductor.org/packages/ release/bioc/html/topGO.html ClusterProfiler v. 4.0.2 Yu et al.76 N/A R 4.1.0 https://www.R-project.org/ INSPIRE software https://www.merckmillipore.com/ IDEAS software https://www.merckmillipore.com/ Kaluza analysis software Beckman Coulter Life Sciences N/A Other HALLMARK OXIDATIVE PHOSPHORYLATION gsea-msigdb.org Somervaille_UP Somervaille et al.26 N/A Somervaille_DOWN Somervaille et al.26 N/A STEMNESS_UP gsea-msigdb.org STEMNESS_ DOWN gsea-msigdb.org GAL_LEUKEMIC STEM CELL_DOWN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_DN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_UP gsea-msigdb.org HALLMARK_TNFA_SIGNALING_VIA_NFKB gsea-msigdb.org HSC Differentiation and Lineage-specific markers pathcards.genecards.org Wnt Signaling (beta-Catenin) pathcards.genecards.org MAPK-Erk Signaling pathcards.genecards.org POSITIVE REGULATION OF CELL CYCLE gsea-msigdb.org Respiratory electron transport_ATP synthesis pathcards.genecards.org FRIDMAN_SENESCENCE_UP gsea-msigdb.org TANG_SENESCENCE_TP53_TARGETS_DN gsea-msigdb.org

    Article Title: Genetic modifications of EGLN1 reactivate HbF production in β 0 -thalassemia/HbE.
    Article Snippet: A total of 2 × 105 HSPCs were transfected with Cas9-sgRNA ribonucleoprotein and maintained in the expansion medium for 48 h. HSPCs were subsequently transferred into erythroid differentiation medium (EDM) consisting of IMDM (SH30259.02; Cytiva, Logan, UT) supplemented with 5%v/v male AB serum, 100 U/ml penicillin and 100 μg/ml streptomycin, 2 mM GlutaMAX, 330 μg/ml human holo-transferrin (Prospec-Tany, Ness Ziona, Israel), 10 μg/ml recombinant human insulin (Prospec-Tany), 2 IU/ml heparin (Leo, Ballerup, Denmark) and 3 IU/ml erythropoietin (Cilag AG, Schaffhausen, Switzerland).

    Article Title: Identification of a Novel Neurotrophic Factor from Primary Retinal Müller Cells Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
    Article Snippet: Human recombinant CXCL10 (100 ng/ml; Prospec, Rechovot, Israel), human Holo-transferrin (1000 ng/ml; Prospec), human recombinant LIF (1 and 100 ng/ml; Prospec), human recombinant CCL5 (2 and 10 ng/ml; Peprotec, Hamburg, Germany), and human recombinant IL8 (10 and 100 ng/ml; Peprotec) in DMEM/F-12 medium (Invitrogen) or medium alone (negative control) were applied to the PR cultures 20 h after preparation.


    Transfection:

    Article Title: IL-21/IL-21R signaling renders acute myeloid leukemia stem cells more susceptible to cytarabine treatment and CAR T cell therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6J Charles River MGI: 3028467 Il21R / (B6.129-Il21rtm1Kopf/J) MGI: 5435248 Il21 / (B6.129S-Il21tm1Lex/Mmucd) MGI: 4843320 Il21mCherry(B6.Cg-Tg(Il21-mCherry)1Wjl/Mmnc) MGI: 5478531 Recombinant DNA Plasmid pMDLg/pRRE Addgene #12259 Plasmid pRSV-Rev Addgene #12253 Plasmid pCMV-VSV-g Addgene #8454 CD19-CAR plasmid Addgene #200671 Software and algorithms FlowJoTM software v.10.6 TreeStar N/A GraphPad Prism software v9.0 GraphPad N/A ELDA Software Hu et al.21 http://bioinf.wehi.edu.au/software/elda/ fastqc v. 0.11.9 https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ RSeQC v. 4.0.0 Wang et al.72 N/A HiSat2 v. 2.2.1 Kim et al.73 N/A FeatureCounts v. 2.0.1 Liao et al.74 N/A DESeq2 v.1.32.0 Love et al.75 N/A TopGo v. 2.44.0 https://bioconductor.org/packages/ release/bioc/html/topGO.html ClusterProfiler v. 4.0.2 Yu et al.76 N/A R 4.1.0 https://www.R-project.org/ INSPIRE software https://www.merckmillipore.com/ IDEAS software https://www.merckmillipore.com/ Kaluza analysis software Beckman Coulter Life Sciences N/A Other HALLMARK OXIDATIVE PHOSPHORYLATION gsea-msigdb.org Somervaille_UP Somervaille et al.26 N/A Somervaille_DOWN Somervaille et al.26 N/A STEMNESS_UP gsea-msigdb.org STEMNESS_ DOWN gsea-msigdb.org GAL_LEUKEMIC STEM CELL_DOWN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_DN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_UP gsea-msigdb.org HALLMARK_TNFA_SIGNALING_VIA_NFKB gsea-msigdb.org HSC Differentiation and Lineage-specific markers pathcards.genecards.org Wnt Signaling (beta-Catenin) pathcards.genecards.org MAPK-Erk Signaling pathcards.genecards.org POSITIVE REGULATION OF CELL CYCLE gsea-msigdb.org Respiratory electron transport_ATP synthesis pathcards.genecards.org FRIDMAN_SENESCENCE_UP gsea-msigdb.org TANG_SENESCENCE_TP53_TARGETS_DN gsea-msigdb.org

    Article Title: Genetic modifications of EGLN1 reactivate HbF production in β 0 -thalassemia/HbE.
    Article Snippet: A total of 2 × 105 HSPCs were transfected with Cas9-sgRNA ribonucleoprotein and maintained in the expansion medium for 48 h. HSPCs were subsequently transferred into erythroid differentiation medium (EDM) consisting of IMDM (SH30259.02; Cytiva, Logan, UT) supplemented with 5%v/v male AB serum, 100 U/ml penicillin and 100 μg/ml streptomycin, 2 mM GlutaMAX, 330 μg/ml human holo-transferrin (Prospec-Tany, Ness Ziona, Israel), 10 μg/ml recombinant human insulin (Prospec-Tany), 2 IU/ml heparin (Leo, Ballerup, Denmark) and 3 IU/ml erythropoietin (Cilag AG, Schaffhausen, Switzerland).

    Article Title: Identification of a Novel Neurotrophic Factor from Primary Retinal Müller Cells Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
    Article Snippet: Human recombinant CXCL10 (100 ng/ml; Prospec, Rechovot, Israel), human Holo-transferrin (1000 ng/ml; Prospec), human recombinant LIF (1 and 100 ng/ml; Prospec), human recombinant CCL5 (2 and 10 ng/ml; Peprotec, Hamburg, Germany), and human recombinant IL8 (10 and 100 ng/ml; Peprotec) in DMEM/F-12 medium (Invitrogen) or medium alone (negative control) were applied to the PR cultures 20 h after preparation.


    Negative Control:

    Article Title: IL-21/IL-21R signaling renders acute myeloid leukemia stem cells more susceptible to cytarabine treatment and CAR T cell therapy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains Mouse: C57BL/6J Charles River MGI: 3028467 Il21R / (B6.129-Il21rtm1Kopf/J) MGI: 5435248 Il21 / (B6.129S-Il21tm1Lex/Mmucd) MGI: 4843320 Il21mCherry(B6.Cg-Tg(Il21-mCherry)1Wjl/Mmnc) MGI: 5478531 Recombinant DNA Plasmid pMDLg/pRRE Addgene #12259 Plasmid pRSV-Rev Addgene #12253 Plasmid pCMV-VSV-g Addgene #8454 CD19-CAR plasmid Addgene #200671 Software and algorithms FlowJoTM software v.10.6 TreeStar N/A GraphPad Prism software v9.0 GraphPad N/A ELDA Software Hu et al.21 http://bioinf.wehi.edu.au/software/elda/ fastqc v. 0.11.9 https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ RSeQC v. 4.0.0 Wang et al.72 N/A HiSat2 v. 2.2.1 Kim et al.73 N/A FeatureCounts v. 2.0.1 Liao et al.74 N/A DESeq2 v.1.32.0 Love et al.75 N/A TopGo v. 2.44.0 https://bioconductor.org/packages/ release/bioc/html/topGO.html ClusterProfiler v. 4.0.2 Yu et al.76 N/A R 4.1.0 https://www.R-project.org/ INSPIRE software https://www.merckmillipore.com/ IDEAS software https://www.merckmillipore.com/ Kaluza analysis software Beckman Coulter Life Sciences N/A Other HALLMARK OXIDATIVE PHOSPHORYLATION gsea-msigdb.org Somervaille_UP Somervaille et al.26 N/A Somervaille_DOWN Somervaille et al.26 N/A STEMNESS_UP gsea-msigdb.org STEMNESS_ DOWN gsea-msigdb.org GAL_LEUKEMIC STEM CELL_DOWN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_DN gsea-msigdb.org BROWN_MYELOID_CELL_DEVELOPMENT_UP gsea-msigdb.org HALLMARK_TNFA_SIGNALING_VIA_NFKB gsea-msigdb.org HSC Differentiation and Lineage-specific markers pathcards.genecards.org Wnt Signaling (beta-Catenin) pathcards.genecards.org MAPK-Erk Signaling pathcards.genecards.org POSITIVE REGULATION OF CELL CYCLE gsea-msigdb.org Respiratory electron transport_ATP synthesis pathcards.genecards.org FRIDMAN_SENESCENCE_UP gsea-msigdb.org TANG_SENESCENCE_TP53_TARGETS_DN gsea-msigdb.org

    Article Title: Genetic modifications of EGLN1 reactivate HbF production in β 0 -thalassemia/HbE.
    Article Snippet: A total of 2 × 105 HSPCs were transfected with Cas9-sgRNA ribonucleoprotein and maintained in the expansion medium for 48 h. HSPCs were subsequently transferred into erythroid differentiation medium (EDM) consisting of IMDM (SH30259.02; Cytiva, Logan, UT) supplemented with 5%v/v male AB serum, 100 U/ml penicillin and 100 μg/ml streptomycin, 2 mM GlutaMAX, 330 μg/ml human holo-transferrin (Prospec-Tany, Ness Ziona, Israel), 10 μg/ml recombinant human insulin (Prospec-Tany), 2 IU/ml heparin (Leo, Ballerup, Denmark) and 3 IU/ml erythropoietin (Cilag AG, Schaffhausen, Switzerland).

    Article Title: Identification of a Novel Neurotrophic Factor from Primary Retinal Müller Cells Using Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
    Article Snippet: Human recombinant CXCL10 (100 ng/ml; Prospec, Rechovot, Israel), human Holo-transferrin (1000 ng/ml; Prospec), human recombinant LIF (1 and 100 ng/ml; Prospec), human recombinant CCL5 (2 and 10 ng/ml; Peprotec, Hamburg, Germany), and human recombinant IL8 (10 and 100 ng/ml; Peprotec) in DMEM/F-12 medium (Invitrogen) or medium alone (negative control) were applied to the PR cultures 20 h after preparation.




    Similar Products

    human holo-transferrin  (ProSpec)
    90
    ProSpec human holo-transferrin
    Human Holo Transferrin, supplied by ProSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+holo-transferrin/human+holo+transferrin/pm39536753-222-133-135
    Average 90 stars, based on 1 article reviews
    human holo-transferrin - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    human holo transferrin  (Merck & Co)
    86
    Merck & Co human holo transferrin
    Human Holo Transferrin, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+holo-transferrin/transferrin/pmc12996415-390-0-2
    Average 86 stars, based on 1 article reviews
    human holo transferrin - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    transferrin  (R&D Systems)
    93
    R&D Systems transferrin
    Transferrin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+holo-transferrin/Human+Holo-Transferrin+Protein%2C+CF/10__1002_slash_adfm__202511627-328-28-29
    Average 93 stars, based on 1 article reviews
    transferrin - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    transferrin r d systems  (R&D Systems)
    93
    R&D Systems transferrin r d systems
    Transferrin R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+holo-transferrin/Human+Holo-Transferrin+Protein%2C+CF/us12421501-200-61-62
    Average 93 stars, based on 1 article reviews
    transferrin r d systems - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    human holo  (R&D Systems)
    93
    R&D Systems human holo
    Human Holo, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+holo-transferrin/Human+Holo-Transferrin+Protein%2C+CF/pmc12395447-219-21-26
    Average 93 stars, based on 1 article reviews
    human holo - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    human holotransferrin  (R&D Systems)
    93
    R&D Systems human holotransferrin
    (A). Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL <t>holotransferrin;</t> (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g). Geometric means (GM) of each population are annotated per facet; (C). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and R 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (D). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (E). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse; (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.
    Human Holotransferrin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+holo-transferrin/Human+Holo-Transferrin+Protein%2C+CF/bio_rxiv__2025__06__22__660918-185-0-2
    Average 93 stars, based on 1 article reviews
    human holotransferrin - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    crystallized human holo-transferrin  (Millipore)
    90
    Millipore crystallized human holo-transferrin
    (A). Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL <t>holotransferrin;</t> (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g). Geometric means (GM) of each population are annotated per facet; (C). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and R 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (D). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (E). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse; (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.
    Crystallized Human Holo Transferrin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+holo-transferrin/crystallized+human+holo+transferrin/bio_rxiv__2025__03__20__644441-93-16-18
    Average 90 stars, based on 1 article reviews
    crystallized human holo-transferrin - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    human holo transferrin  (Beyotime)
    90
    Beyotime human holo transferrin
    (A). Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL <t>holotransferrin;</t> (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g). Geometric means (GM) of each population are annotated per facet; (C). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and R 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (D). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (E). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse; (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.
    Human Holo Transferrin, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+holo-transferrin/annexin+v+fitc+apoptosis+detection+kit/pm39826335-189-0-24
    Average 90 stars, based on 1 article reviews
    human holo transferrin - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    human holo transferrin  (ProSpec)
    90
    ProSpec human holo transferrin
    (A). Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL <t>holotransferrin;</t> (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g). Geometric means (GM) of each population are annotated per facet; (C). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and R 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (D). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (E). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse; (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.
    Human Holo Transferrin, supplied by ProSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+holo-transferrin/human+holo+transferrin/pm39536753-260-33-36
    Average 90 stars, based on 1 article reviews
    human holo transferrin - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A). Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL holotransferrin; (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g). Geometric means (GM) of each population are annotated per facet; (C). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and R 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (D). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (E). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse; (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.

    Journal: bioRxiv

    Article Title: Cell-type specific iron content regulation revealed by single-cell iron quantification

    doi: 10.1101/2025.06.22.660918

    Figure Lengend Snippet: (A). Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL holotransferrin; (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g). Geometric means (GM) of each population are annotated per facet; (C). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and R 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (D). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (E). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse; (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.

    Article Snippet: Human holotransferrin (R&D systems, 2914-HT-001G) was added at concentrations of 0.001 mg/mL to 0.625 mg/mL.

    Techniques: Activity Assay

    (A). Mean fluorescence intensity (MFI) of CD71 (transferrin receptor) versus the geometric mean single cell Fe content (ag) for each population distribution, from each holotransferrin condition; (B). Mean fluorescence intensity (MFI) of CD25 (alpha chain component of the IL-2 surface receptor on T-cells) versus the geometric mean single cell Fe content (ag) for each population distribution, from each holotransferrin condition; (C). Mean fluorescence intensity (MFI) of CD71 versus the geometric mean single cell Ca content (ag) for each population distribution, from each holotransferrin condition; (D). Mean fluorescence intensity (MFI) of CD25 versus the geometric mean single cell Ca content (ag) for each population distribution, from each holotransferrin condition. Error bars present the 95 th confidence intervals for each metallomic distribution determined by sc-ICP-MS.

    Journal: bioRxiv

    Article Title: Cell-type specific iron content regulation revealed by single-cell iron quantification

    doi: 10.1101/2025.06.22.660918

    Figure Lengend Snippet: (A). Mean fluorescence intensity (MFI) of CD71 (transferrin receptor) versus the geometric mean single cell Fe content (ag) for each population distribution, from each holotransferrin condition; (B). Mean fluorescence intensity (MFI) of CD25 (alpha chain component of the IL-2 surface receptor on T-cells) versus the geometric mean single cell Fe content (ag) for each population distribution, from each holotransferrin condition; (C). Mean fluorescence intensity (MFI) of CD71 versus the geometric mean single cell Ca content (ag) for each population distribution, from each holotransferrin condition; (D). Mean fluorescence intensity (MFI) of CD25 versus the geometric mean single cell Ca content (ag) for each population distribution, from each holotransferrin condition. Error bars present the 95 th confidence intervals for each metallomic distribution determined by sc-ICP-MS.

    Article Snippet: Human holotransferrin (R&D systems, 2914-HT-001G) was added at concentrations of 0.001 mg/mL to 0.625 mg/mL.

    Techniques: Fluorescence